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Publish date: 91 / 07 / 11 | Rating: Article Rating

Application Deadline: 31/10/2012

Start of PhD project: Jan 2013 or earlier

Source of Funding: BMBF

Project Description:

Airway inflammation represents a hallmark of major lung diseases including cystic fibrosis, asthma and COPD. Molecular crosstalk between immune and epithelial cells has gained major interest. However precise mechanisms are largely unknown.

We have previously established airway surface dehydration by epithelial dysfunction as a major cause for airway inflammation (1). Moreover we have identified epithelial ion channels critically modulated by immune-derived cytokines (2).  Preliminary data suggest breakdown of immunological tolerance in mice with reduced airway mucus clearance. Peripheral tolerance involves regulatory T cells (3) and other leukocyte subpopulations in the lung.

The PhD student will focus on the cellular basis for immuno-epithelial interactions in vivo and in vitro. The student will unravel alterations of leukocyte subpopulations in an established transgenic mouse model. Epithelial sodium channel overexpression in this mouse model shows severe mucus obstruction (1). Moreover, analysis of immune cells derived factors on epithelial cells in vitro will allow detection of molecular mechanisms. Our preliminary data show promising candidates for further exploration. The project will be embedded in the novel Translational Lung Research Center Heidelberg (University, DKFZ, EMBL) with access to cutting edge technology, weekly journal club, monthly international seminars and active meeting participation.

The Translational Lung Research Center Heidelberg is embedded within the German Center for Lung Research (DZL), a national health center intitiave (http://www.dzg-lungenforschung.de/).

 

References:

(1) Mall M, Grubb BR, Harkema JR, O'Neal WK, Boucher RC. Nat Med. 2004 May;10(5):487-93. Epub 2004 Apr 11.

(2) Anagnostopoulou P, Riederer B, Duerr J, Michel S, Binia A, Agrawal R, Liu X, Kalitzki K, Xiao F, Chen M, Schatterny J, Hartmann D, Thum T, Kabesch M, Soleimani M, Seidler U, Mall MA. J Clin Invest. 2012 Sep

(3) Fritzsching B, Oberle N, Pauly E, Geffers R, Buer J, Poschl J, Krammer P, Linderkamp O, Suri-Payer E. Blood. 2006 Nov 15;108(10):3371-8. Epub 2006 Jul 25.

Methods that will be used:

Flow Cytometry, Mouse Lung Function, Confocal Microscopy, Immunohistology, Cell Culture and basic molecular biology methods (i.e. quantitative PCR, transient overexpression), Electrophysiology

Collaboration Partners within the Translational Lung Reseach Center Heidelberg include:

University Hospital Heidelberg, DKFZ, EMBL

Profile of candidate’s qualification:

Master/diploma or state examination /M.D. degree, excellent cv, basic training in immunology and/or lung biology, flow cytometry, cell culture and molecular biology are desirable. The enthusiastic candidate should be able to rapidly gain project responsibility, be interested in open discussions with colleagues and supervisors and able to present data on international meetings.

 

Applications should include curriculum vitae, a short letter of motivation, copy of important certificates (University, School) and contact addresses of two referees.

 

Applications should be sent by e-mail within 4 weeks to:

Email: Benedikt.Fritzsching@med.uni-heidelberg.de

Department Translational Pulmonology (Head: Prof. Dr. M. mall)

Translational Lung Research Center

University of Heidelberg

Im Neuenheimer Feld 350

69120 Heidelberg

Germany

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  • Airway inflammation â crosstalk between immune cells and airway epithelium -Heidelberg/Germany
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